KineticScan?高内涵筛选分析仪

Key Features

• High Resolution Cellular and Sub-cellular Imaging Monitor and analyze multiple, individual cells with sub-cellular resolution. Data reveals heterogeneity of kinetic responses and enables researchers to make better decisions by observing correlations that are not revealed in well-average responses.
• Kinetic Assay Scheduling Software Permits fully automated, unattended execution of complex, live cell experimental protocols involving data acquisition, processing and analysis, as well as pipetting activities for increased productivity.
• Integrated Liquid Handling System Fully functional liquid handling for compound and reagent delivery, mixing and cell washing during live cell experiments.
• Onboard Environmental Control Enables live cell experiments by maintaining precise physiological conditions throughout the assay time course, resulting in more biologically meaningful results.
• Flexible Optical System Combining a full spectrum light source with automated filters and objectives provides flexibility for a wide variety of fluorescent reporters, including FRET reagents and dyes for ratio metric imaging.
• Proprietary BioApplications Reduces assay development time by providing researchers with “plug and play” software modules and the flexibility to adjust assay parameters for measurement of a wide range of biological outputs.

Live cells were monitored for three separate indicators of cell health. Upon addition of a toxin, the aggregate response of a population of cells exhibits a rapid drop in mitochondrial potential (red), followed by a modest increase and slow decay in cytosolic calcium (green), and a gradual increase in membrane permeability (blue). Analysis of four individual cells (inset graphs) enhances the details of the sequence and timing of events identified in the well-level analysis. In contrast to the average population response, the changes for all three indicators show unique profiles when viewed at the single-cell level.

Expanding list of biological measurements that have been performed on the KSR using either Target Activation or Compartmental Analysis BioApplications:

• Multiple Drug Resistance (MDR)
• Cell Spreading
• Receptor Internalization
• Calcium, Sodium and Potassium Mobilization
• Plasma Membrane Potential
• GFP Expression
• Cytosolic pH
• Lysosomal pH
• Mitochondrial Transmembrane Potential
• Cell Permeability
• Cell Proliferation
• Cytotoxicity
• Oxidative Stress
• Nitric Oxide
• Cell Motility
• GPCR Signaling Events
• Glutathione depletion
• Apoptosis