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Pulsed-Q Dissociation (PQD)
PQD, a new patented fragmentation mechanism, is now available on Thermo's 2D linear ion traps, eliminating the "1/3 Rule" or “Low Mass Cut-Off” for MS/MS data. Use of PQD allows for trapping and analysis of structure-informative ions such as b-1, y-1 and immonium ions. PQD also enables the detection of MS/MS reporter ions from isobaric peptide tags such as iTRAQ.

You can now also order the new ASMS 2006 applications CD, or learn about other mass spectrometry or proteomics solutions from Thermo.

PQD is a novel fragmentation mechanism developed for the Finnigan™ LTQ that involves precursor ion activation at high Q, a time delay to allow the precursor to fragment, then a rapid pulse to low Q where all fragment ions are trapped. The product ions can then be scanned out of the ion trap and detected. PQD fragmentation produces precise, reproducible fragmentation and has been used for iTRAQ peptide quantitation on the Finnigan LTQ using both electrospray and vMALDI source ionization. PQD fragmentation of peptides produces robust precursor ion fragmentation, and provides the ability to routinely scan product ions down to m/z 50. Fragmentation occurs in about the same time scale as Collision Induced Dissociation (CID) and can easily be incorporated into general Xcalibur methods and used in conjunction with collision-induced dissociation (CID), MSn, and other scan functions. Learn more about applications utilizing PQD in the following HUPO 2005 presentation: Analysis of Low Mass Ions in Peptide Fragmentation Spectra With a Linear Ion Trap (393 Kb)iTRAQ is a trademark of Applera Corporation.